![]() ![]() With estimated 636.000 incident cases in the developed countries and 514.000 in the developing countries, breast cancer is the most prevalent cancer type among woman worldwide. In the industrialized countries, breast cancer is the leading cause of death for women at the age between 30 and 60 years. Although every organ in the human body can be afflicted with cancer, there are significant differences in frequency relating amongst others to age, sex, geographic region and personal habits. Cancer is induced by numerous different elicitors, which finally all lead to an interference of the genetically regulated balance between cell cycle and apoptosis. “Cancer” describes a group of various diseases, where cells start changing their molecular structure and begin to grow and to supersede normal cells. ![]() 1.2 RNA Expression Profiling and Predictionġ.3 Conceptional Formulation and ObjectivesĢ.1.2 Buffers and Chemicals for Nucleic Acid PurificationĢ.1.3 Nucleic Acids, Cell Lines and Tissue SamplesĢ.2.3 Creation of a Standard Reference RNA PoolĢ.2.5 Designing Dually Labeled Primer-Probe SetsĢ.2.8 Evaluation of Different Reporter DyesĢ.2.9 Controlling of Primer-Probe-Set PerformanceĢ.2.10 Testing Combinations of Two Sets (Duplexing)Ģ.2.11 Testing Combinations of Three Sets (Triplexing)ģ.1 Identification of Suitable Reporter Dyesģ.2 Evaluation of Primer-Probe-Performanceģ.3 Identification of Suitable Duplex Combinationsģ.4 Identification of Suitable Triplex CombinationsĤ.1 Statistical Evaluation of Primer PerformancesĤ.3 Optimization of Assays and kinetic RT-PCR Parameterħ.5 Origins of Breast Cancer Samples for MAVPOOL080623aħ.6 Acknowledgements 1 Introduction 1.1 Breast Cancer ![]()
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